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Potentially a noninvasive measure of peroxidation in vivo. Results using mammals have been variable; some authors have found that the technique works well, but others have abandoned it. Many GC columns do not separate pentane from isoprene. Ethane can result from free radical attack on certain amino acids (Biochem Pharmacol 1990; 39:1347). Peroxidation of PUFAs produces a complex mixture of isoprostranes. , ∼30–40 pg/mL in fresh human plasma, ∼2 ng/mg creatinine in human urine). Measurement of Peroxides.

Chloroform:methanol mixtures) before analysis is a common approach to this problem. Nevertheless, it is dangerous to assume that diene-conjugated material equates with lipid peroxidation. Separation of the UV-absorbing “diene conjugate” material from human body fluids revealed that most or all of it consists of a non–oxygen-containing isomer of linoleic acid, octadeca-9(cis),11(trans)-dienoic acid. This and similar UV-absorbing products are found in several foods (red meat, meat products, butter, lard, cream, ice-cream, cheese, milk, and yogurt) and can also be produced by bacterial metabolism (192,193).

If a compound appears to inhibit myeloperoxidase, it should be checked whether the compound is an inhibitor or is simply a competing substrate for the enzyme. 2 and using its molar absorption coefficient at Copyright © 2002 by Taylor & Francis Group, LLC 235 nm to calculate the concentration (228). Thus, the ability of a putative antioxidant to protect a target against attack by HOCl can be examined. Targets that have been used include thiols (229) and α1 -antiprotease (230). A good scavenger of HOCl should protect α1 -antiprotease against inactivation when HOCl is added.

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